csm ade Search Results


pnubg ntrac5  (Valiant Co Ltd)
93
Valiant Co Ltd pnubg ntrac5
Left column: medial confocal optical sections through representative pollen tubes expressing the indicated eYFP fusion proteins, or free eYFP used as control, either transiently (CRIB NtGAP1 -eYFP) or stably (all other fluorescent proteins). Numbers (top right) indicate the growth rate (µm/min) of the individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath [eYFP] or at the PM) in all analyzed pollen tubes expressing each indicated protein. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MDs) from the apex (MD = 0 µm). Both sides of imaged pollen tubes were analyzed, if possible. Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal and distal half-maximal RFI (if applicable). A) Intracellular distribution and PM association of <t>eYFP-NtRAC5</t> (n = 49 pollen tubes, 95 RFI distribution patterns), along with the intracellular distribution of free eYFP (n = 55 pollen tubes, 110 RFI distribution patterns). B) Intracellular distribution and PM association of CRIB NtGAP1 -eYFP serving as a marker for GTP-bound active RAC/ROP GTP (n = 61 pollen tubes, 118 RFI distribution patterns), as well as of eYFP fused to NtGEF12a (n = 55 pollen tubes, 110 RFI distribution patterns) or to NtGAP1 (n = 65 pollen tubes, 128 RFI distribution patterns), which activate or inactivate RAC/ROP signaling functions, respectively.
Pnubg Ntrac5, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/csm+ade/bio_rxiv__2024__04__08__588543-334-14-32?v=Valiant+Co+Ltd
Average 93 stars, based on 1 article reviews
pnubg ntrac5 - by Bioz Stars, 2026-08
93/100 stars
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ynb  (Valiant Co Ltd)
96
Valiant Co Ltd ynb
Left column: medial confocal optical sections through representative pollen tubes expressing the indicated eYFP fusion proteins, or free eYFP used as control, either transiently (CRIB NtGAP1 -eYFP) or stably (all other fluorescent proteins). Numbers (top right) indicate the growth rate (µm/min) of the individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath [eYFP] or at the PM) in all analyzed pollen tubes expressing each indicated protein. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MDs) from the apex (MD = 0 µm). Both sides of imaged pollen tubes were analyzed, if possible. Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal and distal half-maximal RFI (if applicable). A) Intracellular distribution and PM association of <t>eYFP-NtRAC5</t> (n = 49 pollen tubes, 95 RFI distribution patterns), along with the intracellular distribution of free eYFP (n = 55 pollen tubes, 110 RFI distribution patterns). B) Intracellular distribution and PM association of CRIB NtGAP1 -eYFP serving as a marker for GTP-bound active RAC/ROP GTP (n = 61 pollen tubes, 118 RFI distribution patterns), as well as of eYFP fused to NtGEF12a (n = 55 pollen tubes, 110 RFI distribution patterns) or to NtGAP1 (n = 65 pollen tubes, 128 RFI distribution patterns), which activate or inactivate RAC/ROP signaling functions, respectively.
Ynb, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/csm+ade/pmc07576474-79-15-24?v=Valiant+Co+Ltd
Average 96 stars, based on 1 article reviews
ynb - by Bioz Stars, 2026-08
96/100 stars
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tryptophan  (Valiant Co Ltd)
92
Valiant Co Ltd tryptophan
Left column: medial confocal optical sections through representative pollen tubes expressing the indicated eYFP fusion proteins, or free eYFP used as control, either transiently (CRIB NtGAP1 -eYFP) or stably (all other fluorescent proteins). Numbers (top right) indicate the growth rate (µm/min) of the individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath [eYFP] or at the PM) in all analyzed pollen tubes expressing each indicated protein. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MDs) from the apex (MD = 0 µm). Both sides of imaged pollen tubes were analyzed, if possible. Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal and distal half-maximal RFI (if applicable). A) Intracellular distribution and PM association of <t>eYFP-NtRAC5</t> (n = 49 pollen tubes, 95 RFI distribution patterns), along with the intracellular distribution of free eYFP (n = 55 pollen tubes, 110 RFI distribution patterns). B) Intracellular distribution and PM association of CRIB NtGAP1 -eYFP serving as a marker for GTP-bound active RAC/ROP GTP (n = 61 pollen tubes, 118 RFI distribution patterns), as well as of eYFP fused to NtGEF12a (n = 55 pollen tubes, 110 RFI distribution patterns) or to NtGAP1 (n = 65 pollen tubes, 128 RFI distribution patterns), which activate or inactivate RAC/ROP signaling functions, respectively.
Tryptophan, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/csm+ade/pmc06810650-120-9-11?v=Valiant+Co+Ltd
Average 92 stars, based on 1 article reviews
tryptophan - by Bioz Stars, 2026-08
92/100 stars
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csm-ade-his-leu-trp-ura  (Valiant Co Ltd)
90
Valiant Co Ltd csm-ade-his-leu-trp-ura
Left column: medial confocal optical sections through representative pollen tubes expressing the indicated eYFP fusion proteins, or free eYFP used as control, either transiently (CRIB NtGAP1 -eYFP) or stably (all other fluorescent proteins). Numbers (top right) indicate the growth rate (µm/min) of the individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath [eYFP] or at the PM) in all analyzed pollen tubes expressing each indicated protein. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MDs) from the apex (MD = 0 µm). Both sides of imaged pollen tubes were analyzed, if possible. Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal and distal half-maximal RFI (if applicable). A) Intracellular distribution and PM association of <t>eYFP-NtRAC5</t> (n = 49 pollen tubes, 95 RFI distribution patterns), along with the intracellular distribution of free eYFP (n = 55 pollen tubes, 110 RFI distribution patterns). B) Intracellular distribution and PM association of CRIB NtGAP1 -eYFP serving as a marker for GTP-bound active RAC/ROP GTP (n = 61 pollen tubes, 118 RFI distribution patterns), as well as of eYFP fused to NtGEF12a (n = 55 pollen tubes, 110 RFI distribution patterns) or to NtGAP1 (n = 65 pollen tubes, 128 RFI distribution patterns), which activate or inactivate RAC/ROP signaling functions, respectively.
Csm Ade His Leu Trp Ura, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/csm+ade/custom%40114550122%4025887987?v=Valiant+Co+Ltd
Average 90 stars, based on 1 article reviews
csm-ade-his-leu-trp-ura - by Bioz Stars, 2026-08
90/100 stars
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csm-ade-his-leu-met-trp-ura  (Valiant Co Ltd)
90
Valiant Co Ltd csm-ade-his-leu-met-trp-ura
Left column: medial confocal optical sections through representative pollen tubes expressing the indicated eYFP fusion proteins, or free eYFP used as control, either transiently (CRIB NtGAP1 -eYFP) or stably (all other fluorescent proteins). Numbers (top right) indicate the growth rate (µm/min) of the individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath [eYFP] or at the PM) in all analyzed pollen tubes expressing each indicated protein. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MDs) from the apex (MD = 0 µm). Both sides of imaged pollen tubes were analyzed, if possible. Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal and distal half-maximal RFI (if applicable). A) Intracellular distribution and PM association of <t>eYFP-NtRAC5</t> (n = 49 pollen tubes, 95 RFI distribution patterns), along with the intracellular distribution of free eYFP (n = 55 pollen tubes, 110 RFI distribution patterns). B) Intracellular distribution and PM association of CRIB NtGAP1 -eYFP serving as a marker for GTP-bound active RAC/ROP GTP (n = 61 pollen tubes, 118 RFI distribution patterns), as well as of eYFP fused to NtGEF12a (n = 55 pollen tubes, 110 RFI distribution patterns) or to NtGAP1 (n = 65 pollen tubes, 128 RFI distribution patterns), which activate or inactivate RAC/ROP signaling functions, respectively.
Csm Ade His Leu Met Trp Ura, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/csm+ade/custom%40114560222%4034541245?v=Valiant+Co+Ltd
Average 90 stars, based on 1 article reviews
csm-ade-his-leu-met-trp-ura - by Bioz Stars, 2026-08
90/100 stars
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csm supplement  (Becton Dickinson)
90
Becton Dickinson csm supplement
Left column: medial confocal optical sections through representative pollen tubes expressing the indicated eYFP fusion proteins, or free eYFP used as control, either transiently (CRIB NtGAP1 -eYFP) or stably (all other fluorescent proteins). Numbers (top right) indicate the growth rate (µm/min) of the individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath [eYFP] or at the PM) in all analyzed pollen tubes expressing each indicated protein. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MDs) from the apex (MD = 0 µm). Both sides of imaged pollen tubes were analyzed, if possible. Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal and distal half-maximal RFI (if applicable). A) Intracellular distribution and PM association of <t>eYFP-NtRAC5</t> (n = 49 pollen tubes, 95 RFI distribution patterns), along with the intracellular distribution of free eYFP (n = 55 pollen tubes, 110 RFI distribution patterns). B) Intracellular distribution and PM association of CRIB NtGAP1 -eYFP serving as a marker for GTP-bound active RAC/ROP GTP (n = 61 pollen tubes, 118 RFI distribution patterns), as well as of eYFP fused to NtGEF12a (n = 55 pollen tubes, 110 RFI distribution patterns) or to NtGAP1 (n = 65 pollen tubes, 128 RFI distribution patterns), which activate or inactivate RAC/ROP signaling functions, respectively.
Csm Supplement, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/csm+ade/pm17014970-76-20-22?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
csm supplement - by Bioz Stars, 2026-08
90/100 stars
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csm-ade-trp-ura  (Valiant Co Ltd)
N/A
Triple dropout formulation (without Adenine, Tryptophan and Uracil) of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations.
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csm-ade-leu  (Valiant Co Ltd)
N/A
Double dropout formulation (without Adenine and Leucine) of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations.
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Image Search Results


Left column: medial confocal optical sections through representative pollen tubes expressing the indicated eYFP fusion proteins, or free eYFP used as control, either transiently (CRIB NtGAP1 -eYFP) or stably (all other fluorescent proteins). Numbers (top right) indicate the growth rate (µm/min) of the individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath [eYFP] or at the PM) in all analyzed pollen tubes expressing each indicated protein. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MDs) from the apex (MD = 0 µm). Both sides of imaged pollen tubes were analyzed, if possible. Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal and distal half-maximal RFI (if applicable). A) Intracellular distribution and PM association of eYFP-NtRAC5 (n = 49 pollen tubes, 95 RFI distribution patterns), along with the intracellular distribution of free eYFP (n = 55 pollen tubes, 110 RFI distribution patterns). B) Intracellular distribution and PM association of CRIB NtGAP1 -eYFP serving as a marker for GTP-bound active RAC/ROP GTP (n = 61 pollen tubes, 118 RFI distribution patterns), as well as of eYFP fused to NtGEF12a (n = 55 pollen tubes, 110 RFI distribution patterns) or to NtGAP1 (n = 65 pollen tubes, 128 RFI distribution patterns), which activate or inactivate RAC/ROP signaling functions, respectively.

Journal: bioRxiv

Article Title: Plasma membrane and cytoplasmic compartmentalization: a dynamic structural framework required for pollen tube tip growth

doi: 10.1101/2024.04.08.588543

Figure Lengend Snippet: Left column: medial confocal optical sections through representative pollen tubes expressing the indicated eYFP fusion proteins, or free eYFP used as control, either transiently (CRIB NtGAP1 -eYFP) or stably (all other fluorescent proteins). Numbers (top right) indicate the growth rate (µm/min) of the individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath [eYFP] or at the PM) in all analyzed pollen tubes expressing each indicated protein. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MDs) from the apex (MD = 0 µm). Both sides of imaged pollen tubes were analyzed, if possible. Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal and distal half-maximal RFI (if applicable). A) Intracellular distribution and PM association of eYFP-NtRAC5 (n = 49 pollen tubes, 95 RFI distribution patterns), along with the intracellular distribution of free eYFP (n = 55 pollen tubes, 110 RFI distribution patterns). B) Intracellular distribution and PM association of CRIB NtGAP1 -eYFP serving as a marker for GTP-bound active RAC/ROP GTP (n = 61 pollen tubes, 118 RFI distribution patterns), as well as of eYFP fused to NtGEF12a (n = 55 pollen tubes, 110 RFI distribution patterns) or to NtGAP1 (n = 65 pollen tubes, 128 RFI distribution patterns), which activate or inactivate RAC/ROP signaling functions, respectively.

Article Snippet: The haploid yeast strains THY.AP4 and THY.AP5 ( ) were transformed with pMetOYC-NtGEF12a or pNubG-NtRAC5, respectively, and plated on appropriate selective media (CSM +Ade, His, Trp, Ura or CSM +Ade, His, Leu; MP Biomedicals) according to .

Techniques: Expressing, Stable Transfection, Fluorescence, Standard Deviation, Marker

A) Alignment of linear bars representing all PM domains associated with various proteins and lipids ( , and ), or in contact with different cytoplasmic structures or regions , which were investigated in this study. Mean meridional distances (MDs) from the pollen tube apex (MD = 0 µm) of the proximal (if applicable) and distal endpoints of these PM domains were quantitatively determined (values indicated next to each bar and in ) and statistically compared using one-way ANOVA with Tukey Kramer (HSD) testing. Error bars: standard deviation. Distinct letters indicate statistically significantly different mean MDs from the apex (p < 0.05). Red vertical lines: maximal RFI indicated in , , and . Number of pollen tubes/domain endpoints analyzed (both sides of pollen tubes were analyzed, if possible): PI4P, n = 70/140; PI4,5P 2 , n = 83/155; DAG, n = 65/130; PS, n = 60/119; PA, n = 81/162; NtPLC3, n = 117/231; NtPI4P-5K4, n = 55/106; NtRAC5, n = 55/107; RAC/ROP GTP , n = 66/132; NtGEF12a, n = 61/122; NtGAP1, n = 67/134; F-actin fringe, n = 63/126; TGN, n = 69/138; VAR, n = 57/114; Ca 2+ , n = 67/134. For direct comparison, the extension of the apical PM domain displaying high membrane order as well as the position of the lateral peak in membrane order are also indicated . The apical PM domain displaying high membrane order is identical to the secretory domain. Light blue shading indicates a PM region in which the endpoints of many apical and lateral domains are positioned. All domains are drawn to scale. B) Left column: medial confocal optical sections through representative pollen tubes stably expressing the indicated fluorescent fusion proteins serving as markers for the following cytoplasmic structures or regions: F-actin (LIFEACT-eYFP), subapical TGN compartment (eYFP-RISAP), VAR (NtINT4-eYFP) or region displaying elevated Ca 2+ concentrations (2xR-GECO1). Numbers (top right) indicate the growth rate (µm/min) of individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath the PM) in all analyzed pollen tubes expressing LIFEACT-eYFP (n = 59 pollen tubes, 110 RFI distribution patterns), eYFP-NtRISAP (n = 58 pollen tubes, 116 RFI distribution patterns), NtINT4-eYFP (n = 55 pollen tubes, 109 RFI distribution patterns) or 2xR-GECO1 (n = 55 pollen tubes, 108 RFI distribution patterns). Both sides of imaged pollen tubes were analyzed, if possible. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MD) from the apex (MD = 0 µm). Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal (if applicable) and distal half-maximal RFI.

Journal: bioRxiv

Article Title: Plasma membrane and cytoplasmic compartmentalization: a dynamic structural framework required for pollen tube tip growth

doi: 10.1101/2024.04.08.588543

Figure Lengend Snippet: A) Alignment of linear bars representing all PM domains associated with various proteins and lipids ( , and ), or in contact with different cytoplasmic structures or regions , which were investigated in this study. Mean meridional distances (MDs) from the pollen tube apex (MD = 0 µm) of the proximal (if applicable) and distal endpoints of these PM domains were quantitatively determined (values indicated next to each bar and in ) and statistically compared using one-way ANOVA with Tukey Kramer (HSD) testing. Error bars: standard deviation. Distinct letters indicate statistically significantly different mean MDs from the apex (p < 0.05). Red vertical lines: maximal RFI indicated in , , and . Number of pollen tubes/domain endpoints analyzed (both sides of pollen tubes were analyzed, if possible): PI4P, n = 70/140; PI4,5P 2 , n = 83/155; DAG, n = 65/130; PS, n = 60/119; PA, n = 81/162; NtPLC3, n = 117/231; NtPI4P-5K4, n = 55/106; NtRAC5, n = 55/107; RAC/ROP GTP , n = 66/132; NtGEF12a, n = 61/122; NtGAP1, n = 67/134; F-actin fringe, n = 63/126; TGN, n = 69/138; VAR, n = 57/114; Ca 2+ , n = 67/134. For direct comparison, the extension of the apical PM domain displaying high membrane order as well as the position of the lateral peak in membrane order are also indicated . The apical PM domain displaying high membrane order is identical to the secretory domain. Light blue shading indicates a PM region in which the endpoints of many apical and lateral domains are positioned. All domains are drawn to scale. B) Left column: medial confocal optical sections through representative pollen tubes stably expressing the indicated fluorescent fusion proteins serving as markers for the following cytoplasmic structures or regions: F-actin (LIFEACT-eYFP), subapical TGN compartment (eYFP-RISAP), VAR (NtINT4-eYFP) or region displaying elevated Ca 2+ concentrations (2xR-GECO1). Numbers (top right) indicate the growth rate (µm/min) of individual pollen tubes shown, which was determined after image acquisition (average growth rate of all analyzed pollen tubes: Supplemental Fig. S1). Scale bars: 8 µm. Right column: quantitative analysis of PM-associated fluorescence (directly underneath the PM) in all analyzed pollen tubes expressing LIFEACT-eYFP (n = 59 pollen tubes, 110 RFI distribution patterns), eYFP-NtRISAP (n = 58 pollen tubes, 116 RFI distribution patterns), NtINT4-eYFP (n = 55 pollen tubes, 109 RFI distribution patterns) or 2xR-GECO1 (n = 55 pollen tubes, 108 RFI distribution patterns). Both sides of imaged pollen tubes were analyzed, if possible. Light green dots: mean relative fluorescence intensity (RFI) associated with the PM at different meridional distances (MD) from the apex (MD = 0 µm). Dark grey vertical lines: standard deviation. Red vertical lines: maximal RFI. Black vertical lines delimiting light grey shading: proximal (if applicable) and distal half-maximal RFI.

Article Snippet: The haploid yeast strains THY.AP4 and THY.AP5 ( ) were transformed with pMetOYC-NtGEF12a or pNubG-NtRAC5, respectively, and plated on appropriate selective media (CSM +Ade, His, Trp, Ura or CSM +Ade, His, Leu; MP Biomedicals) according to .

Techniques: Standard Deviation, Comparison, Membrane, Stable Transfection, Expressing, Fluorescence